title> Secrets of Aids Of Aids Ebola Zika and other man-made diseases 'description'/>”description” content=”Analysis of latest health news and reliable information on Aids, Ebola, Zika virus, the origins of man-made and the deliberate spread of diseases SECRETS OF AIDS, EBOLA, ZIKA VIRUS, AND OTHER MAN-MADE DISEASES

Tuesday, March 21, 2017

REACTION TO WHY TRUMP HAS TO PURGE WHO/CDC/ AND FDA

Polio eradication in Africa gave birth to Aids disease in the continent because the vaccine was contaminated

Polio eradication in Africa gave birth to Aids disease in the continent because the vaccine was contaminated


The writers of this blog have decided to publish comments, opinions, and reactions of our readers. In the past, some comments weren't made available to readers because it didn't occur to us to publish them. Now it's the time to value whatever comment comes from our readers.

Following the article, we published captioned WHY TRUMP HAS TO PURGE WHO / CDC / AND FDA? we had a comment yesterday as published.


Everyone wake up! They have been killing us off one by one for years! Big government does this! Corruption and money hungry Politicians and Big Business moguls! These millionaires don't give a crap about life, except theirs! Pray and support Trump every day! Pray for Robert Kennedy, Jr.

Also because he had a hard road ahead of him! Evil is everywhere in our gov't! So sad! But that's what happens when God is pushed out! Now you know why Following Jesus Christ and being a Christian is very important!

Not only important for saving your soul from hell but also because it fights and cares well-being of all people! Regardless of color or race or status quo! God love all people and unless you know Jesus, then your intentions at some time will turn evil!

America was founded on God and freedom of religion! Christianity doesn't force anyone to believe! It's your choice or not! But Christianity will look out for your rights still! Because we love people! God loves you just as you are!


Without God in our Country, chaos and evil take over! To survive, America must come back to God! Support Trump! It's a God thing!


If you want to share your opinion on any article on this blog, you are always welcome. Send your article to 


THE DEVELOPMENT OF EBOV (ZAIRE KIKWIT STRAIN)



Laboratory-engineered Ebola virus was triggered by the US government killing thousands of people in West Africa. Photo: A man digging the grave to bury an Ebola victim


EBOV (Zaire Kikwit strain) stocks were developed at the USAMRIID with virus originally isolated from an infected patient during the 1995 outbreak and passaged in Vero E6 cells. 

The stock material for the study was obtained in four passages from the original isolate. This stock has been deemed the national stock for preclinical studies for advanced products. 

The stock was made by the Department of Defense Critical Reagents Program under the Joint Program Executive Office for Chemical and Biological Defense. 

The virus and future viruses generated under this program have been developed under the Filovirus Animal Non-Clinical Group (FANG), a multiple-agency group including the U.S. Food and Drug Administration, to prepare for validated/regulated efficacy studies.


NHP challenge and care


Adult male and female rhesus macaque NHPs were caged individually. NHPs underwent surgery for TA10TA-D70 telemetry implantation (DSI). After the NHPs were placed in training jackets (Lomir Biomedical) for acclimatization, which lasted for 6 days.

Surgeries to place central venous catheters (CVCs) (Groshong 7F; Bard) were performed, and adequate recovery time was provided before transfer into biosafety level 4 (BSL4) containment. 

After CVC placement, custom jackets (Lomir Biomedical) were used to contain and protect the CVC lines connected to a mounted swivel system (Instech Solomon). These lines were locked with taurolidine-citrate catheter solution (UNO B.V.) 

After the   NHPs were moved into containment, acclimated, and connected to the swivel system, the lines were flushed at least once daily with PBS (BD) and locked with heparin (BD) to maintain line functionality. 

NHPs were given monkey chow (Harlan), primate treats, fruits, and vegetables for the duration of the study. Before the challenge, NHPs were anesthetized and given a physical examination. 

Animals were challenged intramuscularly with a target dose of 1000 PFU/ml diluted from the stock concentration with minimum essential medium (MEM). After the challenge, the animals were returned to their cages and observed until reasonable mobility was recovered.


Verification of target dose and  viremia

Target challenge doses and viremia were verified by agarose-based plaque assay. Dilution points were serially diluted 10-fold in Eagle’s MEM and adsorbed onto Vero E6 cell monolayers in six-well plates. 

The plates were incubated for 1 hour at 37°C/5% CO2 with rocking about every 15 min, and 2 ml of a 1:1 mixture of medium [2× Eagle’s basal medium with Earle’s salts, 10% (v/v) fetal bovine serum] and 1% agarose (Lonza) was added. 

Plates were then incubated for 7 days at 37°C/5% CO2. After incubation, 2 ml of a 1:1 mixture of the same medium and 4% neutral red stain was added to each well. 

After an additional 24 hours of incubation at 37°C/5% CO2, the plaques were counted, and viremia titers were calculated. Viremia analysis was performed on plasma samples with the same methodology.

MB-003 preparation and treatment Treatments consisted of a mixture of three mAbs, and each mAb was equally represented in the treatment mixture. Treatment for each group was set at 16.7 mg/kg per mAb. 

The treatment for each specific NHP was determined according to the animal’s weight before the challenge. NHPs were treated via intravenous infusion through the CVC with 60-ml syringes (BD) and syringe pumps (Lomir Biomedical) over about 280 min. 

NHPs received MB-003 (50 mg/kg). After the initial positive triggers, all NHPs were treated every 3 days. In total, three in- fusions were given to each  NHP.


Animal monitoring and sample collection

NHPs were monitored for changes in health, diet, behavior, and appearance. Samples used for RT-PCR analysis were collected twice per day at 12-hour increments. If anesthesia was required for the collection samples were only collected once daily in the morning. 

Samples used for CBCs and chemistry analyses were collected on days 0, 3, 5, 7, 10,14, 21, and 28. CBCs were performed with the Hemavet 950 (Drew Scientific), and chemistry analyses were performed with Piccolo 13 general chemistries (Abaxis). 

All blood collections were performed with 1-ml mini collect serum and K3 EDTA plasma tubes (Greiner Bio-One). All sample tubes were centrifuged at 1800g for 10 min, and the resulting material was used or frozen at −80°C for further analysis. 

NHP temperatures were monitored remotely via DSI telemetry every 10 min with the corresponding software (DSI). Experiments were conducted  under  BSL4 containment conditions.


Enzyme-linked immunosorbent assay


ELISAs were performed with a recombinant EBOV glycoprotein (National Cancer Institute), and plasma samples were serially diluted at half-log increments. Goat anti-human IgG (heavy + light) (KPL) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (Millipore) were used as secondary antibodies and substrate, respectively. Assays were evaluated at an absorbance at 405 nm (SpectraMax M5; Molecular Devices).


RNA isolation and  analysis

Qualitative real-time  RT-PCR  (rRT-PCR) was used for the detection of Ebola Zaire virus in plasma samples from exposed NHPs. Samples from the NHPs were inactivated with TRI  reagent  LS (at a ratio of three parts of TRI Reagent LS to one part of plasma). 

RNA in each sample was purified with the Qiagen QIAamp Viral RNA Mini Kit. A volume of 70 ml of inactivated plasma was placed onto the column, and samples were eluted in 70 ml of AVE buffer provided by Qiagen. 

The eluted RNA was run in triplicate with the USAMRIID DSD EZ1 rRT-PCR pre-EUA Assay Kit on the ABI 7500  Fast Dx instrument. This assay kit uses SuperScript II One-Step rRT-PCR sequence-specific primers and a hydrolyzable probe  (TaqMan)  against a conserved region of the Ebola Zaire virus. 

The PCR cycling conditions were as follows: reverse transcription for 15 min at 50°C, Taq activation for 5 min at 95°C, and 45 cycles of amplification with a denaturation step for 1 s at 95°C and an anneal/extension step for 26 s at 60°C. 

Samples were run immediately for qualitative detection at two-time points on days 3 to 5 after infection, with a.m. and p.m. time points where applicable. The sample was considered positive if one of the three replicate reaction was detected with a Ct value or negative if all three replicate reactions were undetected. 

The time required from sample receipt to reportable detection was about  3 hours.


Quantitative RNA isolation and  RT-PCR

RNeasy kits (Qiagen) were used for RNA extraction. One-step quantitative real-time RT-PCRs were performed with a Light Cycler 480 (Roche) in 20-ml volumes with 5 ml of purified RNA using the SuperScript III One-Step RT-PCR System (Invitrogen). 

Primers (forward, 5′-CGGA- CCTGGTTTGGTTGTG-3′;  reverse, 5′-GCTGCAGTGTCGCATCTGA-3′) and TaqMan probe (6-carboxyfluorescein-5′-CCCTTGCCACAATCT- minor groove binder nonfluorescent quencher-3′) (Applied Biosystems) specific for the Ebola Zaire glycoprotein gene were used. 

Program conditions consisted of reverse transcription at 50°C for 20 min and initial denaturation at 95°C for 5 min, followed by 45 cycles of denaturation at 95°C for 5 s; annealing, synthesis, and signal acquisition at 60°C for 20 s; and a final cooling step at 40°C for 30 s. Measurements of viral gene expression were performed with a viral RNA standard.


Statistics

Survival curves were analyzed with the log-rank Mantel-Cox test. For each test, P < 0.05 was considered statistically significant. Survival data are consistent with proportional hazards.

Monday, March 20, 2017

THE ICC WATCH UN ESCAPE PUNISHMENT AFTER MEDICAL CRIME IN HAITI

A clinic in Rendel, Haiti, was overflowing with cholera patients in October. The disease has killed nearly 10,000 people in Haiti since it was introduced there in 2010 by a United Nations peacekeeping force

A clinic in Rendel, Haiti, was overflowing with cholera patients in October. The disease has killed nearly 10,000 people in Haiti since it was introduced there in 2010 by a United Nations peacekeeping force


After Bringing Cholera to Haiti, U.N. Can’t Raise Money to Fight It






When the leader of the United Nations apologized to Haitians for the cholera epidemic that has ravaged their country for more than six years — caused by infected peacekeepers sent to protect them — he proclaimed a “moral responsibility” to make things right.

The apology, announced in December along with a $400 million strategy to combat the epidemic and “provide material assistance and support” for victims, amounted to a rare public act of contrition by the United Nations. Under its secretary-general at the time, Ban Ki-moon, the organization had resisted any acceptance of blame for the epidemic, one of the worst cholera outbreaks in modern times.

Since then, however, the United Nations’ strategy to fight the epidemic, which it calls the “New Approach,” has failed to gain traction. A trust fund created to help finance the strategy has only about $2 million, according to the latest data on its website. Just six of the 193 member states — Britain, Chile, France, India, Liechtenstein and South Korea — have donated.

Other countries have provided additional sources of anti-cholera funding for Haiti outside the trust fund, most notably Canada, at about $4.6 million, and Japan, at $2.6 million, according to the United Nations. Nonetheless, the totals received are a fraction of what Mr. Ban envisioned.

In a letter sent to member states last month, Mr. Ban’s successor, António Guterres, asked for financial commitments to the trust fund by March 6. He also appeared to raise the possibility of a mandatory dues assessment if there were no significant pledges.

The deadline came and went without much response.

Mr. Guterres has not stated publicly whether he intends to push for a mandatory assessment in the budget negotiations now underway at the United Nations. Privately, however, diplomats and United Nations officials said he had shelved the idea, partly because of strong resistance by some powerful members, including the United States.

Diplomats said part of the problem could be traced to simple donor fatigue, as well as to many countries’ reluctance to make financial commitments without certainty that the money will be used effectively.

The donor challenge was acknowledged by Dr. David Nabarro, a United Nations special adviser who rose to prominence running its mobilization to fight the Ebola crisis in West Africa, and who has been leading its fund-raising efforts for Haiti as he seeks to become the next director-general of the World Health Organization.

“Donors will respond, but they need to be convinced that they’re going to be given a good proposition for what’s done with their money,” he said in January at the World Economic Forum. “The Haiti cholera story is not actually a very good one, in that it’s taken us a rather long time to get on top of it, and still the problem is persisting.”

The fund-raising effort has been further complicated by the Trump administration’s intention to cut spending on foreign aid. The United States, historically a leading source of Haiti’s foreign aid, is also the biggest single financing source for the United Nations, which may now confront painful choices over how to allocate reduced revenue.

Ross Mountain, a veteran United Nations aid official who is its senior adviser on cholera in Haiti, said that a number of ideas concerning the financing were under discussion. And, he said, while “$400 million is not a very large sum, considering the circumstances, we are all very aware of the competing demands.”

Mr. Mountain also conceded that “on the financial side, we have not moved further ahead.”

Mr. Trump’s new United Nations ambassador, Nikki R. Haley, who has called the cholera crisis “nothing short of devastating,” did not respond to requests for comment about the funding problem. But in her Senate confirmation testimony in January, Ms. Haley said, “We’re going to have to make this right with Haiti, without question, and the U.N. is going to have to take responsibility.”

Cholera, a waterborne bacterial scourge that can cause acute diarrhea and fatal dehydration if not treated quickly, has killed nearly 10,000 people and sickened nearly 800,000 in Haiti, the Western Hemisphere’s poorest country since it was introduced there in 2010 by infected Nepalese members of a United Nations peacekeeping force. This year, as of late February, nearly 2,000 new cases had been reported, amounting to hundreds a week.

Studies have traced the highly contagious disease to sloppy sanitation that had leached fecal waste laced with cholera germs from latrines used by the Nepalese peacekeepers into the water supply.

We still have the biggest outbreak of cholera of any country anywhere,” said Dr. Louise Ivers, a senior policy adviser at Partners in Health, an international medical aid organization that has long worked in Haiti. “Here we are, nearly seven years later, and it’s still a big problem.”

Compared with other disasters confronting the United Nations, like the Syria refugee crisis and famines threatening 20 million people in Yemen and parts of Africa, the Haiti crisis may not loom as large. But unlike the others, the direct cause in Haiti was traced to the United Nations.

This fact weighed on Mr. Ban until near the end of his tenure. He finally acted after the organization’s independent investigator on extreme poverty and human rights, Philip Alstonsaid in a scathing report that the United Nations’ failure to take responsibility for the cholera crisis was “morally unconscionable, legally indefensible and politically self-defeating.”

But Mr. Ban’s apology for Haiti’s cholera epidemic also clearly reflected an assumption that all members were responsible for the success of the new strategy to defeat it. “For the sake of the Haitian people, but also for the sake of the United Nations itself, we have a moral responsibility to act,” he told the General Assembly on Dec. 1. “And we have a collective responsibility to deliver.”

Advocacy groups that had been somewhat heartened by Mr. Ban’s words have grown increasingly anxious not only about the lack of money but also about the lack of clarity in how the “material assistance and support” part of the plan, which represents half of the $400 million goals will be used.

Two leading advocacy groups for Haitian cholera victims, the Bureau des Avocats Internationaux and the Institute for Justice and Democracy in Haiti, sent a letter on Thursday to Mr. Guterres, requesting a meeting and expressing concern that “the current trajectory of fund-raising and elaboration of the New Approach is betraying the U.N.’s promises of a meaningful and accountable response in Haiti.”

Lawmakers in the United States critical of the United Nations’ response in Haiti have also put pressure on the organization.

“While the U.N. has admitted to wrongdoing and promised to create a fund to provide restitution to the people of Haiti victimized by cholera,” Representative John Conyers Jr., Democrat of Michigan, said in a statement last week, “they have failed to make good on these promises.”

Opinion of the writers of this blog

Why those responsible for medical crimes in Third World Countries go unpunished?  If one commits a crime and apologizes, still he has to be punished else people will kill every day and be set free after the apology.

Thus, the United Nations should have faced criminal charges as soon as they apologized for being responsible for the cholera epidemic that has claimed over ten thousand people in Haiti. This is a broad daylight medical crime that the Haitians need justice.

Medical crimes committed by the United Nations, World Health Organization and Centers for Diseases Control passed unnoticed because those responsible are either 'above' the law or the victims are poor so they deserve it.

When the Ebola bio-weapon was triggered by the American government in Sierra Leone, Guinea, and Liberia, health officials deliberately delayed for the victims to die. When this unprofessional attitude came to light and was published on our blog, the World Health Organization quickly apologized.

Reference: EBOLA COULD HAVE BEEN STOPPED BY THE END OF 2014

While the Ebola victims were dying daily in large numbers, all international flights to the affected countries were cancelled by Europe and America. But when Teresa Romero tested positive for the disease it drew more than 375,000 signatures. 

This was a disease which was deliberately done. It didn't miraculously appear from heaven because of poverty. Despite the innocent lives that were lost, the International Criminal Court did nothing about this. The criminals responsible now enjoy impunity. Yet, they put African leaders behind bars for committing a crime against humanity.

The World Health Organization, Centers for Diseases Control and the United Nations have ruined their reputation to the extent that there is nothing they can do to pacify the souls of those deliberately murdered. They are health institutions without conscience and regrets. 

Their cups will be full one day even if it takes thousand years for little drops of water to make a mighty ocean.